Example of unknown bacteria lab report. Unknown project lab report 4 2022-12-27
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An unknown bacteria lab report is a document that describes the process of identifying an unidentified bacterial sample. This type of report is typically used in microbiology labs, where scientists and students are often tasked with identifying unknown bacteria as part of their coursework or research.
To identify an unknown bacterial sample, a number of techniques and tests must be used. These techniques can include physical and chemical characteristics of the bacteria, as well as its growth patterns and ability to survive in different environments.
One example of an unknown bacteria lab report might involve the identification of a bacterial sample that was collected from a patient with a respiratory infection. The sample would be grown in a laboratory setting and observed for its physical characteristics, such as shape, size, and color. The sample might also be tested for its ability to survive in different temperatures, pH levels, and other conditions.
To further identify the bacterial sample, the lab might use biochemical tests to determine the presence or absence of certain enzymes or other chemical compounds. For example, the lab might test the sample for the production of certain enzymes that are characteristic of certain bacterial species, such as lactase or urease.
Once all of the data has been collected and analyzed, the lab can use a combination of physical, chemical, and biochemical characteristics to identify the unknown bacterial sample. The lab report would then include a detailed description of the identification process, as well as any relevant information about the identified bacteria, such as its potential to cause disease and how it might be treated.
In conclusion, unknown bacteria lab reports are an important tool for identifying and understanding the characteristics and behavior of different bacterial species. These reports provide valuable information that can be used to better understand and treat bacterial infections, and they are an essential part of the scientific process.
Unknown sample Lab Report blog.sigma-systems.com
Getting a positive result from the capsule stain, I next performed the mannitol salt agar MSA test. All of the test results came out correct, matching all the characteristic of E. The purple color of the stain indicates that the species is Gram positive. The purpose of this lab was to use different techniques to identify two unknown species of bacteria, one being gram-negative and the other gram positive. A green color indicates vigorous fermentation. With this, it was possible to eliminate all Citrobacter, Enterobacter, Escherichia, Proteus, Salmonella, Shigella, Serratia, Klebsiella and Morganella species as it can be observed in Figure 1. My preliminary identification for the known unknown was S.
Bacterial isolation and identification are important in the medical industry to identify pathogens isolated from a patient. It is commonly known as being harmful, but this is not always the case. Clinical Infectious Diseases, 32 4 , 605-610. The pathogenic cell produces slime, which is secreted from the teichoic acid in the cell wall, resulting in a biofilm formation. Based on those results, I concluded that my Gram-positive isolate was catalase positive, and was therefore either Staphylococcus or Micrococcus. MATERIALS AND METHODS The lab instructor gave out a test tube labeled number 110 with two unknown bacterium. RESULTS — flowcharts were removed from here for formatting reasons CONCLUSION Several test were performed to conclude the gram negative bacteria was Escherichia coli and the gram positive bacteria was Staphylococcus epidermidis.
By knowing which agents are causing a disease, one can determine the correct treatment necessary with persons afflicted with the microbes in question. Throughout the study the course laboratory manual was periodically referenced to perform the techniques properly. From the NA plates, a tiny portion of the well-isolated bacterias was collected with a sterile loop and streaked using the quadrant streak technique on either the MSA or EMB plates. In addition, I was feeling sick and had a sore throat when taking samples and suspected that may influence the bacteria in my oral cavity. St Louis: St Louis Community College at Meramec, 2011. The differential tests Unknown Report On Microbiology And The Epidemiology Of An Organism Kelsey Nolte Microbiology Otero Junior College Mr.
The IMViC test showed no ability to hydrolyse tryptophan, large amounts of acid products, and ability to use citrate as a sole carbon source. After rinsing the dye off with water, iodine was applied to the smear for one minute in order to lock in the dye. The General Microbiology I course taught students about many kinds of bacteria. The known unknown was indeed urease positive, confirming my preliminary identification. Additional Methyl Red test was conducted to verify the result. The second test performed was a Urea Test which also gave a clear positive result confirming that unknown gram positive bacteria as Staphylococcus epidermidis because Staphylococcus aureus gives a negative result.
The gram stain procedure was performed as directed in the lab manual 1. Identifying the unknown organisms is significant meaning for human beings to maintain a healthy environment, prevent infectious diseases, conduct biomedical researches, and develop new antibiotic drugs. It showed negative result. However, I found that S. This semester, several biochemical tests and techniques were provided and could be used to identify two unknown bacteria from 101 test tube. Even on an MSA agar, it took a few tries and several isolations to get the gram positive to successfully grow. Hospitals have high rates of E.
The next step was to amplify the 16s RNA gene fragment, as this gene fragment is highly conserved and is present in almost all bacteria. A bacteria smear was prepared and first stained with crystal violet for 30 seconds. Assuming all the tests were performed correctly, and that the specific strains our group had were largely consistent, these several tests should independently point to one organism. I confirmed this was the Gram-positive bacteria with the gram stain. UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture.
Lab Report Example: Classification of the Unknown Bacterias
If protein was broken down by gelatinase the tube appeared to have white substances in the tube. Some of these toxins are incredibly fast acting and can cause symptoms in as little as thirty minutes after ingestion. VP and Caselin tests were used for testing gram positive bacteria. The next step on the flow chart was to test for lactose fermentation. The problem encountered was that the contamination of isolated Gram positive nutrient agar from MSA. Once incubated and growth formed, isolation of the Gram positive bacterium was confirmed via Gram Stain.
Results Test Gram Stain Purpose To determine the Gram reaction of the organism and identify the morphology Oxidase Test To determine the presence of cytochrome c Methyl Red Test To determine if the organism ferments glucose through mixed acid fermentation VogesTo determine if Proskauer Test the organism performs 2,3butanediol fermentation MacConkey Test To determine if the organism ferments lactose into acid end products Reagents Crystal Violet, Iodine, Alcohol, Safranin Observations Pink Rods Oxidase Reagent No color change Methyl Red reagent No color change Reagent a and Ethanol Reagent b Potassium Hydroxide and Water Lactose, Bile Salts, Crystal Violet, Neutral Red No color change Pink growth on media Result Gram Negative, bacteria Oxidase test negative no cytochrome c Negative Methyl Red test no mixed acid fermentation Negative VogesProskauer test no acetoin production through 2,3butanediol fermentation Positive MacConkey Test Lactose fermentation Discussion After performing all of my test, the results from each test was gathered and interpreted. The gram negative bacteria was streaked on the surface of the Simmons citrate agar and was incubated. Before starting the unknowns project our group performed a knowns project, which means we knew which organism we had each individual had their own organism and we performed several tests on them and recorded the results as well as write a description if it was necessary. For this test, the isolated Gram negative bacterium was streak inoculated onto the agar plate and incubated. Scientist use Bacillus subtilis in order to promote genetic research and due to its highly genetic manipulability levels, it is easier to conduct testing on Swartzburg, 2009.
When identifying them in laboratory characteristics such as gram staining, Endospore staining, motility, and isolation are used as identification and isolation methods. According to McDonald et al. To confirm Staphylococcus epidermidis is the unknown gram positive bacteria a mannitol test was inoculated and incubated. The problem was solved. The purpose of this study was to identify the two different bacterium from a mixed culture, one Gram positive and one Gram negative. While this is helpful in a classroom setting, this luxury is not always present in the real world. In order to attain correct identification of my two unknown bacterium I had to perform a series of experiments on them, known as biochemical tests.
