Gram stain lab report sample. Microbiology Gram Stain Lab Report [jlk9vkrv6045] 2022-12-27
Gram stain lab report sample
Rating:
8,2/10
1506
reviews
Law is a vast and complex field with a wide range of topics that can be studied and researched. There are numerous issues that can be explored within the realm of law, and there are many ways in which these topics can be approached. Below are just a few examples of potential research paper topics in law:
Cybercrime: With the increasing reliance on technology and the internet, cybercrime has become a significant issue. Researching topics such as cyberbullying, identity theft, and online scams can help shed light on this growing problem and suggest potential solutions.
Environmental law: Environmental law deals with the protection of the environment and natural resources. Researching topics such as climate change, pollution, and wildlife conservation can help inform policies and practices that promote sustainability and protect the planet.
Human rights: Human rights are a critical aspect of the legal system and are designed to protect individuals from harm and discrimination. Researching topics such as racial discrimination, gender equality, and religious freedom can help identify and address areas where human rights are being violated.
Intellectual property: Intellectual property law deals with the protection of creative works, such as inventions, trademarks, and copyrights. Researching topics such as patent law, trademark law, and copyright law can help understand the legal framework surrounding intellectual property and its impact on society.
International law: International law is a complex and rapidly evolving field that deals with the legal relations between nations and international organizations. Researching topics such as war crimes, international trade, and human rights violations can help shed light on important global issues and suggest potential solutions.
There are many other potential research paper topics in law, and the best topic for a research paper will depend on the interests and goals of the researcher. No matter what topic is chosen, it is important to approach it with an open mind, a willingness to learn, and a desire to make a positive impact on society.
Microbiology Gram Stain Lab Report [jlk9vkrv6045]
Then, use a pipette to remove the substrate from the bottles and into centrifuge tubes. In addition, students will receive their AHA Cards the day of class! Antibiotic discs was placed on the surface of the medium. Bacteria are a large group of one-celled organisms. After that measurement, a small amount of antiseptic is applied onto the E. He help to distinguish Klebsiella pneumonia to pneumococci.
Next
Gram Stain Test Lab Report
Safranin is a negative stain, while Crystal violet is a positive stain. The heat sets the proteins of the organism thus causing the bacteria to attach to the slide. How is a Gram stain test performed? This test gave a positive result turning a brown color, meaning the gram negative bacteria produced the enzyme casease in order to break down the milk protein casein. The groups of patients that are at greatest risk of acquiring Citobacter infections are immunocompromised patient and neonates. Gram stain is still the cornerstone method that is used in identifying and finding the taxonomic division of bacteria. Finally, smear was ready to be stained and we move on to the second method in gram staining Method two In method two we first place the prepared and heat-fixed smear on the staining rack, that we prepared during method one. In the second step we flood the smear with Crystal Violet for 60 seconds.
Next
Bacteria Gram Stain Lab Report
In this Lab we will be using a series of dyes that will result in some bacteria staining purple and others staining pink. Lab Manual for General Microbiology. The smear was rinsed with water to remove the alcohol that was in excess. The gram stain procedure was performed as directed in the lab manual 1. Gram stains are usually scanned for the presence or absence of white blood cells indicative of infection and squamous epithelial cells indicative of mucosal contamination.
Next
Gram Stain Purpose, Procedure, Results and more
Then, observing the morphological characteristics of bacteria, under a brightfield microscope, of the Citrobacter freundii a gram positive bacteria and the Staphylococcus aureus a gram negative bacteria. Learning the gram staining techniques have helped us understand how bacteria can be identify for proper treatment and for future studies of bacterial infections. Gram-negative will appear to have direct cross-linking of peptidoglycan meanwhile Gram-positive will have interbridge transpeptidation. All of the biochemical tests performed were explained, in the lab manual provided by Professor 1 and were practiced earlier in the semester. The first step to figuring out the unknowns, was to separate the two bacteria. Furthermore, bacteria are transparent and cannot be seen through the microscope. A purple color was also observed on the addition of Crystal violet to the smear of E.
Next
Lab Report Simple Stain Gram Stain
These bacteria must be able to be identified in order to treat patients properly, efficiently and safely. The first step in Gram staining was to place the primary stain, Crystal Violet or Methylene Blue, on the slide where the bacteria were heat fixed. After getting a good gram stain the identification tables were referred to in order to choose between appropriate biochemical tests. Testing schedules may vary. The importance of using the gram staining procedure is related to the choice of treatment for the patient. The purple color was retained by S. Gram stain procedural steps.
Next
Example of a Microbiology Unknown Lab Report
This dye has no effect on Gram-positive cells, which remain purple. Clendening 2014 … Unknown Lab Report An unknown bacterium was handed out by Dr. After washing the smear, it was covered with a Counterstain, the solution of Safranin, and left to stand for two minutes. The differential tests used to identify the unknown cultures were Gram stain, Catalase, Mannitol Salt Agar MSA , Blood Agar, Novobiocin, Coagulase, and DNAse Alachi, 2007. Basic stains are applied to bacterial smears that have been heat-fixed. After inoculation, visible colonies may or may not be present.
Next
Lab Report 2
Escherichia coli Procedure: 1. Results Through the process of the experiment, unknown number 20 which was on a Nutrient Agar slant medium which was determined to be cocci sphere shaped appeared to be Gram-negative. The structural variations found in the cell walls of bacteria will determine the color of the stain; blue positive or red negative. Gram staining procedure At the lab, a medical laboratory scientist smears or spreads the sample on glass microscope slides. Proteins are next tested. Gram stain is a differential stain because it uses more than two dyes as opposed to one dye in a single stain.
Next
Gram Staining Lab Report Discussion Essay Example
The first step was adding one drop of sterile water on the slide by the use of a sterile inoculating loop to prepare a smear mixture of E. Here is some information about it. The researcher was to use trypan blue exclusion method to test for cell viability, observe the non-viable and viable cells, and was able to have a cell count using the Agglutination Test Lab Report 1772 Words 8 Pages This can be tested by simply mixing the serum of suspected individual which contain the antibodies with the antigens of specific bacteria the accumulation of clumps confirms the presence of particular bacterial infection. The gram stain of M. The Gram-positive bacteria S. The thickness of the Gram-positive wall is 20-80 nanometres, while Gram-negative has a wall thickness of 8-10 nanometres Tankeshwar, 2015, p7. Again the slide was rinsed with deionized water and blotted dry.
Next
Gram Staining Lab Report Essay Essay
Gram stain is the most valuable diagnostic test in bacterial meningitis that is immediately available. Gram-positive bacteria have more of this polymer than gram-negative bacteria. Draw the cells showing morphology, grouping, and relative sizes, Color a few of the cells of each bacterial species to show the Gram reaction. Not all of the tests were performed on every culture, however, as some of the tests were used only for gram + or gram – bacteria, while others were even more specific and used only for cocci bacteria. Unknown Bacteria Lab Report 1034 Words 5 Pages Bacteria can be classified as gram positive or negative difference in call wall. Organism isolation and identification will usually be performed only if culture is requested.
Next
008540: Gram Stain
I use the aseptic treatment while doing this making sure to flame the inoculating loop before and after transferring bacteria. I observed that organism B was non-motile and did not produce endospores. When it came down to narrowing the organisms down to ours, I looked at the glucose test first because all of the other Gram negative organisms were positive for glucose except for two. After observation, a sample was taken from the isolated colony on the streak plate and another streak plate was done with that, trying to further isolate the colonies. My bacterium was beta hemolytic as well.
Next