Gram stain lab report sample. Microbiology Gram Stain Lab Report [jlk9vkrv6045] 2022-12-27

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Microbiology Gram Stain Lab Report [jlk9vkrv6045]

gram stain lab report sample

Then, use a pipette to remove the substrate from the bottles and into centrifuge tubes. In addition, students will receive their AHA Cards the day of class! Antibiotic discs was placed on the surface of the medium. Bacteria are a large group of one-celled organisms. After that measurement, a small amount of antiseptic is applied onto the E. He help to distinguish Klebsiella pneumonia to pneumococci.

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Gram Stain Test Lab Report

gram stain lab report sample

Safranin is a negative stain, while Crystal violet is a positive stain. The heat sets the proteins of the organism thus causing the bacteria to attach to the slide. How is a Gram stain test performed? This test gave a positive result turning a brown color, meaning the gram negative bacteria produced the enzyme casease in order to break down the milk protein casein. The groups of patients that are at greatest risk of acquiring Citobacter infections are immunocompromised patient and neonates. Gram stain is still the cornerstone method that is used in identifying and finding the taxonomic division of bacteria. Finally, smear was ready to be stained and we move on to the second method in gram staining Method two In method two we first place the prepared and heat-fixed smear on the staining rack, that we prepared during method one. In the second step we flood the smear with Crystal Violet for 60 seconds.

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Bacteria Gram Stain Lab Report

gram stain lab report sample

In this Lab we will be using a series of dyes that will result in some bacteria staining purple and others staining pink. Lab Manual for General Microbiology. The smear was rinsed with water to remove the alcohol that was in excess. The gram stain procedure was performed as directed in the lab manual 1. Gram stains are usually scanned for the presence or absence of white blood cells indicative of infection and squamous epithelial cells indicative of mucosal contamination.

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Gram Stain Purpose, Procedure, Results and more

gram stain lab report sample

Then, observing the morphological characteristics of bacteria, under a brightfield microscope, of the Citrobacter freundii a gram positive bacteria and the Staphylococcus aureus a gram negative bacteria. Learning the gram staining techniques have helped us understand how bacteria can be identify for proper treatment and for future studies of bacterial infections. Gram-negative will appear to have direct cross-linking of peptidoglycan meanwhile Gram-positive will have interbridge transpeptidation. All of the biochemical tests performed were explained, in the lab manual provided by Professor 1 and were practiced earlier in the semester. The first step to figuring out the unknowns, was to separate the two bacteria. Furthermore, bacteria are transparent and cannot be seen through the microscope. A purple color was also observed on the addition of Crystal violet to the smear of E.

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Lab Report Simple Stain Gram Stain

gram stain lab report sample

These bacteria must be able to be identified in order to treat patients properly, efficiently and safely. The first step in Gram staining was to place the primary stain, Crystal Violet or Methylene Blue, on the slide where the bacteria were heat fixed. After getting a good gram stain the identification tables were referred to in order to choose between appropriate biochemical tests. Testing schedules may vary. The importance of using the gram staining procedure is related to the choice of treatment for the patient. The purple color was retained by S. Gram stain procedural steps.

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Example of a Microbiology Unknown Lab Report

gram stain lab report sample

This dye has no effect on Gram-positive cells, which remain purple. Clendening 2014 … Unknown Lab Report An unknown bacterium was handed out by Dr. After washing the smear, it was covered with a Counterstain, the solution of Safranin, and left to stand for two minutes. The differential tests used to identify the unknown cultures were Gram stain, Catalase, Mannitol Salt Agar MSA , Blood Agar, Novobiocin, Coagulase, and DNAse Alachi, 2007. Basic stains are applied to bacterial smears that have been heat-fixed. After inoculation, visible colonies may or may not be present.

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Lab Report 2

gram stain lab report sample

Escherichia coli Procedure: 1. Results Through the process of the experiment, unknown number 20 which was on a Nutrient Agar slant medium which was determined to be cocci sphere shaped appeared to be Gram-negative. The structural variations found in the cell walls of bacteria will determine the color of the stain; blue positive or red negative. Gram staining procedure At the lab, a medical laboratory scientist smears or spreads the sample on glass microscope slides. Proteins are next tested. Gram stain is a differential stain because it uses more than two dyes as opposed to one dye in a single stain.

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Gram Staining Lab Report Discussion Essay Example

gram stain lab report sample

The first step was adding one drop of sterile water on the slide by the use of a sterile inoculating loop to prepare a smear mixture of E. Here is some information about it. The researcher was to use trypan blue exclusion method to test for cell viability, observe the non-viable and viable cells, and was able to have a cell count using the Agglutination Test Lab Report 1772 Words 8 Pages This can be tested by simply mixing the serum of suspected individual which contain the antibodies with the antigens of specific bacteria the accumulation of clumps confirms the presence of particular bacterial infection. The gram stain of M. The Gram-positive bacteria S. The thickness of the Gram-positive wall is 20-80 nanometres, while Gram-negative has a wall thickness of 8-10 nanometres Tankeshwar, 2015, p7. Again the slide was rinsed with deionized water and blotted dry.

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Gram Staining Lab Report Essay Essay

gram stain lab report sample

Gram stain is the most valuable diagnostic test in bacterial meningitis that is immediately available. Gram-positive bacteria have more of this polymer than gram-negative bacteria. Draw the cells showing morphology, grouping, and relative sizes, Color a few of the cells of each bacterial species to show the Gram reaction. Not all of the tests were performed on every culture, however, as some of the tests were used only for gram + or gram – bacteria, while others were even more specific and used only for cocci bacteria. Unknown Bacteria Lab Report 1034 Words 5 Pages Bacteria can be classified as gram positive or negative difference in call wall. Organism isolation and identification will usually be performed only if culture is requested.

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008540: Gram Stain

gram stain lab report sample

I use the aseptic treatment while doing this making sure to flame the inoculating loop before and after transferring bacteria. I observed that organism B was non-motile and did not produce endospores. When it came down to narrowing the organisms down to ours, I looked at the glucose test first because all of the other Gram negative organisms were positive for glucose except for two. After observation, a sample was taken from the isolated colony on the streak plate and another streak plate was done with that, trying to further isolate the colonies. My bacterium was beta hemolytic as well.

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