Microbiology lab practical review. microbiology lab practical review parker chiropractic Flashcards 2022-12-12

Microbiology lab practical review Rating: 9,5/10 387 reviews

A microbiology lab practical is a hands-on learning experience that allows students to test their knowledge and understanding of microorganisms and microbial techniques. These lab practicals are an important part of a microbiology course and can be a valuable tool for reinforcing key concepts and skills.

In a microbiology lab practical, students typically work with a variety of microorganisms, including bacteria, viruses, fungi, and protists. They may also use a variety of techniques and equipment to identify and study these microorganisms, such as microscopes, culture media, and biochemical tests.

One of the key objectives of a microbiology lab practical is to teach students how to identify microorganisms based on their physical and biochemical characteristics. This may involve observing microorganisms under a microscope and noting their shape, size, and other physical features. It may also involve performing tests to identify specific metabolic activities or enzymes that are unique to particular microorganisms.

Another important aspect of a microbiology lab practical is the use of aseptic technique. This involves taking steps to prevent contamination of the microorganisms being studied, as well as preventing the spread of infections. This may involve using sterile equipment and techniques, such as flame sterilization, to prevent the introduction of unwanted microorganisms into the lab environment.

In addition to identifying and studying microorganisms, a microbiology lab practical may also involve working with microbial cultures. This may involve preparing and sterilizing culture media, as well as transferring and growing microorganisms in these media. Students may also be asked to perform various tests on these cultures, such as determining the pH or oxygen requirements of a particular microorganism.

Overall, a microbiology lab practical is a valuable learning experience that allows students to apply their knowledge of microorganisms and microbial techniques in a hands-on setting. By working with a variety of microorganisms and using a variety of techniques and equipment, students can gain a deeper understanding of the diversity and complexity of the microbial world.

Lab Practical Review blog.sigma-systems.com

microbiology lab practical review

The purpose of a gram stain is to divide microorganisms into gram positive or gram negative. Unknown labs are performed for many reasons ranging from figuring out the causative agent. Purpose: The purpose of this lab was to learn how to properly mount a bacteria for examination under a microscope. In addition, the tube 2 have pellicle, a layering of growth, of cells at the top. Also learned in this lab were methods. Cover with Safranin Counter Stain and wait 45 seconds 8. The plates are incubated overnight and the zone of inhibition of growth is used to measure susceptibility.

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microbiology lab practical review parker chiropractic Flashcards

microbiology lab practical review

Distinguish between fungal growth and bacterial growth as seen on plates. . The purpose of this experiment was to examine the different g. Air dry for a few minutes and DO NOT BLOT Observe under oil immersion and record results 1. Purpose: The purpose of this lab was to learn how to properly mount a bacteria for examination under a microscope. Rinse slide with distilled water until stain is removed 4.

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Microbiology lab practical 1 study guide

microbiology lab practical review

Blot with Bibulous paper and observe under oil immersion. Once the pathogen is in pure culture, a standardized inoculum equivalent to a 0. . . . The evolution of bubbles oxygen from a 3% hydrogen peroxide solution after a bacterial colony is submerged in the solution indicates the presence of catalase positive result.

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Microbiology Lab Practical Exam Flashcards

microbiology lab practical review

The reaction develops within 15 to 30 seconds. Gram Negative cells turn re. Remove slide with clothes pin and place on staining tray, removing paper towel 7. Blot dry with Bibulous paper, observe under oil immersion and record results 1. Know the types of stains done in the lab or observed during the staining exercises, how they are.

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Microbiology Lab Practical I_Fall blog.sigma-systems.com

microbiology lab practical review

Know what they look like. Compare nutrient agar and Sabouraud dextrose agar SDA with respect to composition and the type of organism whose growth is favored. Place paper towel square on top of smear area 5. The tube is incubated at 55-60 degrees celsius for 48 hours. In this lab, the main. . LAB PRACTICAL 1 REVIEW CONCEPTS Introduction Lab Rules Exercise 1 — Ubiquity of Bacteria Three basic shapes morphologies associated with bacteria Types of arrangement of bacterial cells e.

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Microbiology Practical Review

microbiology lab practical review

. Heat fix bacterial smear 2. Tilt the plate until you can see the elevation of the colonies. Terms: growth medium, agar, aseptic technique. Know the parts of the microscope and their functions.

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Microbiology Lab Practical Review Flashcards

microbiology lab practical review

Know the laboratory safety rules and procedures, proper disposal of contaminated substances, proper procedure for entering and leaving the lab, labelling of cultures, proper attire, BSL levels etc. Autoclave tape is usually placed on objects to be sterilized. . Optochin, bacitracin, and novobiocin are impregnated into paper discs which are used to determine sensitivity to one of these compounds. Meaning more light goes into the lends and therefore better numerical aperture. Cover with Gram's Iodine Mordant and wait for 60 seconds 5.

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MICROBIOLOGY

microbiology lab practical review

Place slide on beaker and let steam for 5 minutes 4. The ampule containing the glucose nutrient broth is crushed by pinching the vial, thereby releasing the broth onto the strip containing the endospores. . Go over your lab handouts in detail. Define the terms in each lab exercise. Rinse with 20% Copper Sulfate Solution Decolorizer and counter stain 5.

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Microbiology Lab Practical

microbiology lab practical review

In the process of Gram staining, a small amount of the cultured bacteria was fixed on a slide with heat. . . When used with the use of an ultra-violet light, it stimulates the spread of germs, teaching how quickly and broadly germs can be spread in a short period of time -Stresses the importance of hand-washing even under fingernails!!! Know how to correctly use a microscope. Also learned in this lab were methods. . Gram + have multiple layers of pepidoglycan in the cell wall which trap the dye, crystal violet.

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