Unknown bacteria lab report. Identification of Unknown Bacteria Lab blog.sigma-systems.com 2022-12-25

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An unknown bacteria lab report is a document that presents the results of an experiment in which the identity of a bacterial sample is determined. The purpose of this experiment is to familiarize students with the techniques used to identify and classify bacteria, as well as to understand the importance of accurately identifying bacteria in a variety of settings, including medical, industrial, and environmental.

The first step in an unknown bacteria lab report is to obtain a bacterial sample. This can be done through a variety of methods, including culturing bacteria from a patient, collecting samples from the environment, or obtaining a pure culture from a laboratory supply company. The sample should be handled and transported in a manner that prevents contamination, as this can affect the accuracy of the results.

Once the sample has been obtained, it is important to prepare it for testing. This may involve diluting the sample, plating it onto agar media, or performing other steps to ensure that the bacteria are viable and able to grow.

Next, a series of tests are conducted to determine the identity of the unknown bacterial sample. These tests may include biochemical tests, such as the metabolism of glucose or the production of certain enzymes, as well as physical tests, such as the shape and size of the bacteria or the presence of certain structures. The results of these tests are recorded and used to narrow down the possible identities of the bacterial sample.

Finally, the results of the tests are analyzed and compared to known bacterial species in order to determine the identity of the unknown sample. This process may involve consulting reference materials, such as bacterial identification guides or online databases, and may require further testing to confirm the identification.

Once the identity of the unknown bacterial sample has been determined, the lab report should summarize the methods and results of the experiment, as well as provide a discussion of the implications of the findings. This may include a discussion of the characteristics and behavior of the identified bacteria, as well as its potential impact on human health or the environment.

In conclusion, an unknown bacteria lab report is a document that presents the results of an experiment to identify and classify a bacterial sample. This experiment is important for understanding the techniques used to identify and classify bacteria, as well as the importance of accurately identifying bacteria in various settings. By carefully following proper protocols and analyzing the results of various tests, it is possible to accurately determine the identity of an unknown bacterial sample.

Example of a Microbiology Unknown Lab Report

unknown bacteria lab report

Unknown Bacterial Identification Lab Report 7 PURPOSE: The purpose of this lab was to identify two unknown bacteria. After properly gram staining the slides as directed in chapter six of the lab manual, the smears were examined under Unknown Bacteria Lab Assignment The purpose of the unknown bacteria lab assignment was to select an unknown bacteria culture and, through a series of metabolic tests, identify which bacteria genus resided in the pure culture received. Kenyon College, 30 June 2006. The experiment was done by applying methods in order to identify an unknown bacterium. An unknown bacterium was handed out by Dr. Methods The gram stain was used to indicate whether each bacterium was gram-positive or gramnegative.

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Unknown Bacteria: Microbiology Lab Report

unknown bacteria lab report

Additionally, researchers in a lab setting must be able to do this in order to further develop their research in whichever ways necessary. However, it was apparent that I had successfully isolated two different bacterial species by examining my MAC and CNA plates. Please note that due to formatting issues the flow charts had to be removed. How can an unknown sample of bacteria be identified? The next test performed was a Nitrate Test which gave a positive result. A few tests that microbiologists will perform are isolation, staining reactions, biochemical reactions, indole tests, Catalase test, Coagulase test, Oxidase test and many others. Professor Snaric advised to do another MSA agar from the original stock, but this time with a sterile swab instead of an inoculating loop. Results Morphology Streptococcus Morphology Singular Rod Gram-positive Hemolytic Beta Gram-negative EMB Urease +++, green sheen Catalase - Bile- esculin Citrate - For the gram-positive cocci species, the tests allowed the identification of a bacteria that is biochemically unable to produce catalase, unable to live in the presence of bile, and highly able to lyse red blood cells.

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How do you write an unknown bacteria in a lab report?

unknown bacteria lab report

The purpose of this lab was to identify two unknown bacteria from a mixed culture. I also performed a cytochrome oxidase test on both organisms; again, organism A was oxidase positive while organism B was oxidase negative. Louis Community College, 2011. Then I decided I would run the biochemical tests on organism B. Some other ways include, having physical contact, contact with animals and high fiber diets 2. In this experiment, an unknown bacterium was to be identified using a number of different tests using several different bacteria for comparison.

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Lab Report Example: Classification of the Unknown Bacterias

unknown bacteria lab report

No red color formation after 15 to 20 minutes is a negative result. The Gram test concluded that the bacterium was a Gram negative rod. Third, even though it might not protect you completely, wash vegetables as thoroughly as possible. Upon returning and observing the streak plate, there was an abundance of green across the plate. A Methyl Red test was performed and had negative results. It is very useful in knowing its risk of toxicity to humans or animals, its resistance or susceptibility to antibiotics, and determining how to control its growth or kill it altogether.

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(Sample) Unknown Lab Report

unknown bacteria lab report

These results correlate with the Escherichia coli species. After isolating the second bacteria, a Gram stain was performed, hoping to see a purple color, indicating a gram positive. Destruction of red blood cells. What is the importance of identifying unknown bacteria? The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics to other purposes such as knowing the exact microorganism has to be used to make certain foods. The starch and milk agar showed a negative indication of amylase and protease respectively. Hemolytic bacteria able to degrade the proteins and red blood cells for metabolic use. Then it was important to record the number of the unknown bacterium as well as the temperature at which the bacteria would be incubated.

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How to Write a Microbiology Unknown Lab Report

unknown bacteria lab report

Currently, there are no treatment options that can cure E. Only one type of colony was evident. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics. UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture.

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unknown bacterium lab report

unknown bacteria lab report

The hemolytic activity test uses a blood enriched medium to indicate hemolytic activity of bacteria. So here is a great paper that might help you along when you are at this point in your studies. The correct plates were chosen based on the shape and color of the bacteria under the microscope. By performing a gram stain on the two colonies I was positive they were two different species because organism A were gram negative rods in single arrangement and organism B were gram positive cocci arranged in irregular clusters. Pseudomonas aeruginosa is a gram negative rod shaped bacteria that was first discovered in 1882 by a pharmacists named Carle Gessard 2.

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Unknown Bacteria Lab Report

unknown bacteria lab report

The gram stain showed a result of red, gram negative rods. A change in the color to red indicates urea degradation. This identification was reached by only one biochemical test and close observation. This left three bacteria that the second unknown could be Staphylococcus aureus, Staphylococcus epidermidis, or Enterococcus faecalis. This experiment was done by applying methods in order to identify an unknown bacterium.

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Unknown Bacterial blog.sigma-systems.com

unknown bacteria lab report

Lab Manual for General Microbiology. In addition, students will receive their AHA Cards the day of class! Because they were growing right next to each other, it was very possible to pick up both bacteria. What is the purpose of the unknown bacteria lab? It is important for people to understand why it is beneficial for us, and even more important to understand how it is harmful. This narrowed down the unknown to 5 possible answers: Escherichia coli, Klebesiella pneumoniae, Enterobacter aerogenes, Proteus vulgaris, and Pseudomonas aeruginesa. The Gram stain revealed it was Gram negative rods. This MSA plate was incubated at 37 degrees Celsius for 48 hours.

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Unknown Bacteria Lab Report

unknown bacteria lab report

Lab day 2; we collected our BHIA medium and began by identifying the morphology and cell-to cell arrangements of the colonies. His study picked up on the unique blue-green pigmentation of P. A lab report is broken down into eight sections: title, abstract, introduction, methods and materials, results, discussion, conclusion, and references. Gives all cells a purple-black colour b. Repeat rinsing after each chemical is added to the slide. The Urea test also had a negative result, which confirmed that the unknown 1 is Escherichia coli. Culture 2 appeared to be solid white dots spread out on the plate.

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