Unknown salt lab. Klebsiella pneumoniae Unknown Lab Report 2022-12-14

Unknown salt lab Rating: 4,7/10 1910 reviews

An unknown salt lab is a common experimental setup in chemistry classrooms and labs. The goal of the lab is for students to identify an unknown salt by performing a series of tests and observations. These tests can include physical properties such as solubility and melting point, as well as chemical reactions with other compounds.

The unknown salt lab is a valuable learning experience for students because it allows them to practice the scientific method and problem-solving skills. By following a set of procedures and using their knowledge of chemistry, students must think critically and make observations to identify the unknown salt. This process helps students understand how chemists identify and characterize substances in the real world.

To begin the lab, students are given an unknown salt and a set of known compounds to use for comparison. They should start by performing physical tests on the unknown salt, such as measuring its solubility in various solvents and determining its melting point. These physical properties can provide clues about the identity of the salt.

Next, students can perform chemical tests on the unknown salt by reacting it with other compounds and observing the results. For example, they could add a drop of the salt solution to a flame and observe the color of the flame. Different salts produce different flame colors, which can help narrow down the possibilities.

Another chemical test that students can perform is a precipitation reaction, in which they add a known compound to the unknown salt solution and observe whether a solid precipitate forms. The presence or absence of a precipitate, as well as the color and shape of the precipitate, can give further clues about the identity of the salt.

Once students have gathered all of their observations and data, they can use this information to narrow down the possible identities of the unknown salt. They should then be able to make a hypothesis about the identity of the salt and use their knowledge of chemistry to support their hypothesis.

Overall, the unknown salt lab is a fun and educational way for students to practice their chemistry skills and apply the scientific method. It helps students understand how chemists identify and characterize unknown substances and encourages them to think critically and solve problems.

Lab 4

unknown salt lab

The addition of a few drops of NaOCl solution, swirled gently as the drops made contact with the unknown created a brown-yellow …show more content… The observation of chemical behaviours under specific conditions allowed for the use of process of elimination. Due to a fact that the salt was exposed to air, it may have resulted in some of the salt reacting with the atmosphere. Furthermore in order to determine how to complete the lab, we needed information upon how to properly keep the temperature of the water from decreasing or increasing, and this required that we needed to know what an insulator is and how to use it. Indicative of Escherichia coli. Klebsiella pneumoniae is a Gram negative rod bacterium that can form a capsule. Hold your diluted tube and the 0.

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Determination of Unknown Salt Experiment

unknown salt lab

Take another sample and try diluting it with deionized water. Our first week of testing, we began our experiment by observing the unknown compounds physical properties, which included state and color. However, sucrose is insoluble in dichloromethane because organic molecules are soluble in organic solvents, and dichloromethane is an Unknown Salt Test The objective of the experiment was to identify the unknown salt that was given. The other ions did not give any specific flame test colour. The community benefits from studies on bacteria because it can advise them on how to keep their families healthy. Secondly, we used a graduated cylinder and measured 25. The concentration of each solution was then calculated by multiplying the amount of moles in the original solution by the amount of original solution used in L then dividing Separation Phases Of The World known that Panacetin contains 8-12% sucrose, 35-45% aspirin, and 45-55% of another unknown active ingredient, but if counterfeited, this active ingredient would most likely be acetanilide or phenacetin.

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Determination of an Unknown Salt 2

unknown salt lab

This makes it difficult to tell whether a precipitate is really white, or just cream or yellow that is too pale to be sure. Objective The objective of this lab is to use the procedures from the previous anion and cation quantitative analysis to identify the anion and cation present in the unknown salt that is given at the beginning of the experiment. Beta hemolysis is indicative of S. The simmons citrate agar turned blue which determined a positive result. Centrifuge and decant the solution from the solid. Since the flame test showed no observable difference this allowed for the conclusion that the cation was Mg2+. A zone of precipitated bile may also be present due to this localized drop in pH.

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EXPERIMENT 3 identification of an unknown blog.sigma-systems.com

unknown salt lab

Since the Gram positive bacterium was identified, one desoxycholate agar plate was streaked from the initial unknown culture to allow growth of only the Gram negative bacterium. Incubate 24-48 hrs at 37°C. Other materials needed in order to complete the lab included paper towels. Solutions in test tubes K-4 and K-6 are both strong acids which turned blue litmus paper to blue. After determining the value of that, we divide it by the theoretical value and multiply it all by 100%. Table 2: Ways to re-dissolve the precipitate Observations for BaCl2 Observations for MgSO A. According to table 1 the data shows that the unknown substance had similar characteristics to both sugar and dirt.

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A Experiment On Unknown Salt Solution

unknown salt lab

Then with the information of the known substances was compared with the characteristics of the unknown substances. Make sure to stir well until the salt and sugar dissolve to see how each substance reacts with water 6 Add vinegar to all substances. Water was required to dissolve our salt into and measure the temperature of. Both MgOH and KSO4 are soluble in water so it would not form a precipitate and it would stay in the solution. Remember, molarity remains constant, while volume changes for each beaker. When taking the measurements of the temperature of both the water and solution, there could have been an error from what we saw and what we wrote. During this experiment many salts will be used.


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Unknown Identification

unknown salt lab

The unknown salt contained an anion and a cation labelled, 38A. The affect this had on our results and solution is a medium result. Given the observations found in the materials and methods section and the data found in the lab manual1 it was found that the anion was not acetate or carbonate, but rather a halide, specifically Br due to the brown-yellow hue of the organic layer. In my glucose fermentation test was positive or acid with no gas in the Durham tube. This is an outline of the required steps to undertake one of these methods.


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Identifying Unknown Salts with Calorimetry

unknown salt lab

After through precipitation, the mixture was placed in drawer to cool down. Lastly, we measured the 3. Add 6M HCl drop- wise until the solution until precipitate forms, or until the solution tests acidic on litmus paper Procedure to separate Iron II as hydroxides 1. Next learn to calibrate the glassware. Enterics The Gram negative enterics are important both as natural flora in the intestinal tract and as pathogens of disease in the gastrointestinal tract and other sites. Be careful not to drop the beakers so that there will be no danger of accidently stepping on glass.

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Lab_6_Unknown_Copper_blog.sigma-systems.com

unknown salt lab

The objectives are: First review use of electronic balance. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. According to the unknown chart, it was concluded that the bacterium was Klebsiella pneumoniae. Solubility rules were also a major theme as solubility is important in determining whether a reaction will produce a precipitate. Hypothesis: Find the unknown substance by testing it out by heating it, pouring water, vinegar, and observing it. Journal of Colloid and Interface Science. Lab Procedure We have included the basic procedure for doing many common biochemical tests below.

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Unknown Salt Lab Chem 1b blog.sigma-systems.com

unknown salt lab

There were three agar plates; one was incubated Unknown Lab Report Essay Unknown Lab Report April 25th, 2006 Introduction The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. Stab the center of the tube to within 3-5 mm of the bottom. A simmons citrate and urea test were performed on the pure culture. Perhaps if we left the solutions together longer, their reaction would have produced a color. Stir the solution 3. A nutrient agar plate was streaked with the unknown culture and incubated at 37 degrees Celsius. Incubate loosely-capped 24-48hrs at 37°C.

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Unknown Mixtures Lab Report

unknown salt lab

The solubility of this precipitate increases when the sample is diluted with deionized water. A series of preliminary tests were first conducted in order to help choose which confirmation tests should be used to identify the compound. By using these observations, identify the unknown ion. The cold pack experiment lab that was conducted by my group and I, had resulted in us facing errors such as measurement errors, errors including the calorimeter and errors including our unknown salt. Results A positive reaction is indicated by obvious turbidity in the media with or without a color change.

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