Catalase is an enzyme that is found in nearly all living organisms and plays a crucial role in the breakdown of hydrogen peroxide, a toxic byproduct of cellular metabolism. In this lab report, we will explore the factors that can affect the activity of catalase, including temperature, pH, and substrate concentration.
To begin, we prepared a solution of hydrogen peroxide and added a small piece of potato, which contains catalase, to the solution. We then measured the amount of oxygen gas produced over a period of time, as the breakdown of hydrogen peroxide by catalase releases oxygen gas as a byproduct. This served as our control experiment, in which we measured the baseline activity of catalase under standard conditions.
Next, we conducted a series of experiments to test the effect of temperature on catalase activity. To do this, we prepared a series of hydrogen peroxide solutions at different temperatures and added potato pieces to each one. We found that catalase activity increased as the temperature increased, up to a certain point. Above this optimal temperature, the activity of the enzyme began to decrease.
We also tested the effect of pH on catalase activity by preparing hydrogen peroxide solutions at different pH levels and adding potato pieces to each one. We found that catalase activity was highest at a pH of around 7, and decreased as the pH increased or decreased from this optimal level.
Finally, we tested the effect of substrate concentration on catalase activity by preparing solutions of hydrogen peroxide at different concentrations and adding potato pieces to each one. We found that catalase activity increased as the substrate concentration increased, but eventually reached a plateau as the concentration of hydrogen peroxide became too high for the enzyme to effectively break down.
Overall, our results showed that temperature, pH, and substrate concentration can all have an effect on the activity of catalase. Understanding these factors can help us to better understand the role of this important enzyme in cellular metabolism and its potential applications in biotechnology.
Lab Report Enzyme Catalase Essay Essay
As a matter of fact, in the absence of normal functioning enzymes, the human body would cease to exist because chemical reactions that are required to maintain the body function would not occur fast enough. Hydrogen peroxide is a regular product in many cellular reactions, but the substance can be toxic, and therefore needs to be broken down to conserve the cells. Background Information: An enzyme is a globular protein that serves as a catalyst for biochemical reactions. The solution turned a bright yellow color after the hydrobromic acid was added. In this experiment we tested the hypothesis that as temperature increases the rate of the chemical reaction decreases, and when the substrate concentration increases the rate of the chemical reaction increases.
The last data point using the 20% enzyme concentration takes much longer to break down the substrate than the other averages, most likely because this set of trials was performed with 3% hydrogen peroxide. The 3 concentrations of enzymes were 0. Performed Catalase Test The Catalase test is performed on organisms to see if it can produce the enzyme catalase. Therefore, the catalase reaction can only forgo if there is both a substrate, and ideal pH level. A general overview is that, ideally, many small samples of the liver would be extracted and similarly to this experiment, the samples would be placed within the substrate, with the denaturing acids slowly added to the solution to yield the results.
The solutions were stirred and the steps for part two were repeated using different volumes of water and hydrogen peroxide, as shown on the table. In the lab, a catalase enzyme was tested on different materials, potato and liver samples. The steps were repeated three times and recorded. Cuvettes 1, 8, and 10 are used as blanks to zero out the spectrophotometer. The higher the concentration of potato extract, or catalase, the faster the reaction and the more substrate present, the faster the reaction.
Controlled Variables temperature, amount of substrate sucrose present, sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity 1. Peroxidase Chemical Reaction Lab 740 Words 3 Pages Title: Enzymes Abstract: Enzymes can catalyze chemical reactions by speeding up the chemicals activation energy. In procedure 2, the results and data show that a pH of 7 is the optimal pH for this reaction to occur. Enzymes accelerate the rate of chemical reactions. I selected Copper Sulphate as enzyme inhibitor against the concentration of 2% of Amylase solution. There is a narrow range of conditions in which enzyme activity is at its peak.
If catalase were to be studied further in future experiments, it is suggested that the catalase specific to bovine liver be experimented with rather than the catalase of a potato, as there would be larger quantities of catalase in mammals. This increased the reliability of the results as it allowed the rate of diffusion of the sulphuric acid into the agar cubes to be calculated more accurately. Controlled Variables pH, amount of B-Galactosidase Lab Report 1764 Words 8 Pages Enzymes speed up chemical reactions enabling more products to be formed within a shorter span of time. The results from the procedure testing temperature conditions differ from what is already known about this topic. Peroxidase Lab 224 Words 1 Pages The purpose of this study is to investigate the effects of varying the concentration of peroxidase on rate of reaction, as well as, the varying temperature and pH levels. They aid in the acceleration of chemical reactions by binding to another substance known as a substrate, converting it into different products that are more easily broken down called products. This was then repeated with the next three samples.
Molecules are what forms when atoms bond together. Enzymes also known as biological catalase, originate from a living organism in order to act as a catalase to boost Catalase Enzyme Activity Lab Report Abstract The purpose of this experiment was to record catalase enzyme activity with different temperatures and substrate concentrations. It is involved in the breaking down of certain substances and the diminish of reactive oxygen species and that includes hydrogen peroxide H2O2 which can be a byproduct of the As with all …show more content… The standard assay was prepared with a 9 Potato Enzyme Lab Report 1182 Words 5 Pages Introduction In class, a series of experiments were performed that pertained to the enzyme known as catalase, which converts hydrogen peroxide into oxygen. At high temperatures, an enzyme denatures or changes shape, making it difficult or impossible for a substrate to bind, and at low temperatures, the frequency and rate of reaction decreases causing for a halt in product formation Pitzer et. Glass 4 involved adding two tablespoons of vinegar, further lowering the pH level in comparison to the third glass. Effects of pH, Ionic Strength, and Temperature on Activation by Calmodulin and Catalytic Activity of Myosin Light Chain Kinase. The organelle responsible for the destruction of hydrogen peroxide is peroxide using the enzyme catalase.
We hypothesized that a higher level of salinity would increase the enzyme activity causing the yeast spheres to rise up faster to the surface while a lower level would be slower in returning the spheres. Our first experiment demonstrated that as the substrate concentration increases the rate of the chemical reaction increases. Catalase is a specific type of enzyme that breaks down Hydrogen Peroxide into water and oxygen. Then submit your finished lab report as a Microsoft Word document. The catalase sample for today's lab will be from a potato. Enzymes are biological catalysts made of proteins that accelerate chemical reactions by lowering their activation energy therefore increasing the activity rate of the enzyme and more substrates turned into products. It works at a biocatalyst that will catalyzing the chemical reaction, which helps to speed up the chemical reaction.
The second procedure is in regard to pH. Studying the effect of temperature and substrate concentration on enzyme concentration allows better understanding of optimum conditions which enzymes can function. Aside from temperature and pH, enzyme concentration and substrate concentration are also important factors to consider for this lab 1. These results match findings in other professional studies. The reaction that will be investigated in this experiment is the decomposition of hydrogen peroxide:2 H2O2 liquid -- Catalase 2 H2O liquid + O2 gas When a catalase enzyme is added to a hydrogen peroxide solution, it becomes water and oxygen gas. Results: The data was collected in this experiment, first, by completing the experimental procedures for the work done with various catalase concentrations and then recording the data and finding the averages. For this experiment, the enzyme catalase will be used.