Cheating in high school can have serious consequences for both the individual who cheated and for the school community as a whole.
First and foremost, cheating undermines the integrity of the educational system and devalues the accomplishments of those who have earned their grades honestly. When students cheat, they are not only cheating themselves out of a genuine education, but they are also taking credit for the hard work and knowledge of others. This can create a sense of unfairness and resentment among those who have put in the effort to earn their grades, and can lead to a breakdown of trust within the school community.
Additionally, cheating can have long-term consequences for the individual who engages in it. Students who cheat may not be adequately prepared for the rigors of college or the workplace, as they have not developed the skills and knowledge necessary to succeed. This can lead to academic and professional failure in the future.
Furthermore, cheating can have legal consequences, especially if it involves the use of electronic devices or other forms of technology. In some cases, students may face criminal charges for hacking into school systems or forging documents.
Finally, cheating can have social consequences, as it can damage a student's reputation and relationships with peers and teachers. Students who are caught cheating may face disciplinary action from the school, including detention, suspension, or even expulsion. This can have a negative impact on their ability to graduate and move on to post-secondary education or employment.
In conclusion, cheating in high school can have serious and long-lasting consequences for both the individual who cheated and the school community as a whole. It is important for students to understand the importance of honesty and integrity in their academic pursuits, and to work hard to earn their grades through their own efforts.
Bacteria Gram Stain Lab Report
As cultures get aged, specifically in Gram-positive bacteria, they always lose their ability to reserve the primary stain and therefore become Gram- variable. In the third step, we gently emulsify the organism in the drop of liquid spreading the mixture over the entire surface of slide to produce an even thin film of bacterial smear. Gram-positive cell wall contains a thick layer of In aqueous solutions, crystal violet dissociates into CV+ and Cl — ions that penetrate through Gram-positive and Gram-negative cell walls. The method and techniques used. Once two minutes have passed, tilt the slide and drain off the excess methanol and let the slide air dry. Safranin is a counter stain use to color the thin peptidoglycan walls of the gram-negatives pink that the de-colorizer striped of crystal violet. This blog shares information and resources about pathogenic bacteria, viruses, fungi, and parasites.
Therefore, in order for them to be successfully obser ved, bacteria need to be prepared. In order for a specimen to be fixed, a thi n film of material containing the microorganism is spread over the surface of the slide, called a smear. The water fills the space between the cover slip and the slide. Title: Gram Stein Technique. Providers usually request bacteria culture tests alongside Gram stain tests.
The other tests that we performed on our unknown were not really helpful when it came to determining if it was Alcaligenes faecalis or Pseudomonas aeruginosa because they both had all the same characteristics when it came to the lactose, mannitol, motility, indole, citrate, H2S, urease, methyl red and VP tests. For the purpose of this… Unknown Lab Report I think we correctly identified our unknown as P. Get a small cuvette. Similarly, only a single type of colony had grown on my CNA plate, indicating a gram positive species, which I chose to be organism B. Immediately wash with water.
The organic solvent such as acetone or ethanol extracts the blue dye complex from the lipid-rich, thin-walled gram-negative bacteria to a greater degree than from the lipid-poor, thick-walled, gram-positive bacteria. Find information and process for the Principle of Gram Stain Image 2: Cell wall of Gram-positive and Gram-negative Bacteria The differences in Gram-positive and Gram-negative bacteria cell wall composition account for the Gram staining differences. PROCEDURE to be done individually 1. Chapter 25 Rocks and Minerals… Gram Staining Lab Prokaryotes are a large group of organisms with no membrane bound organelles. After the minute and a half was up, the slide was rinsed with deionized water and blotted dry.
A positive vogues proskauer test indicates fermentation to neutral products and will turn the broth red, if no color presents, it is also negative. Both Gram-positive and Gram-negative bacteria become directly stained and appear purple after this step. When should I call my doctor? Hypothesis The purposes of this lab is to examine how the Gram Stain techniques are uses by microbiologists to observe the morphological characteristics of bacteria. Variations in Gram Reaction Various factors influence the results of Gram staining. Bacterial cell walls contain a polymer called peptidoglycan.
Gram Stain: What It Is, Purpose, Procedure & Results
A medical laboratory scientist applies the sample to microscope slides, applies a series of stains and looks at the sample under a microscope. Thus, receiving a pinkish reddish color appearance on the heat smear glass slide, under the microscope on 10x objective lens. Almost all bacteria are described by their gram stain characteristics: color, shape and size, and arrangement. We also learned what can go wrong when using gram staining that could produce errors in the testing. These slides are known as smears. Introduction: Most microorganisms, including bacteria, appear colorless when viewed through a light microscope.
Gram-positive bacteria is characterized by having a thick layer of peptidoglycan, called a capsule. Because the cell walls of Gram-negative cells have a higher content of lipids and a thinner layer of peptidoglycan, the alcohol used in the decolorizing step made the Gram-negative cells incapable of retaining the methylene blue-iodine complex. Place one drop of water on the slide. This thin layer of peptidoglycan cell wall makes Gram-negative bacteria to pick up or change to pink on the addition of counterstain Safranin during Gram staining. Gram staining bacteria requires the use of aseptic technique to ensure the sterility of the experiment. When stained with the safranin, the gram-negative bacteria will be stained pink.
Safranin is a negative stain, while Crystal violet is a positive stain. Gram staining works by differentiating bacteria by the chemical and physical properties of their cell walls. Lastly, the saffarin dye was placed on the slide to counterstain the Gramnegative bacteria for one minute, rinsed with deionized water, and blotted dry. Ethyl alcohol was sprayed onto slide, only hitting above the smear, then clothespin was rinsed. Crystal violet is a basic dye that adheres to the cell walls of both gram-positive and gram-negative bacteria, making them both appear purple.
. The next step was to use 95% Ethanol to wash off the dyes from the Gram-negative bacteria. The third difference is that Gram-positive have teichoic acid, while Gram-negative has no teichoic acid. Shake off the excess copper sulfate solution and immediately blot dry. The Gram-positive or negative staining cannot be applied in Eukaryotes since they have no peptidoglycan. The water on the slide helps to support the organism and sample.
