Sporosarcina morphology. Sporosarcina 2022-12-27

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Sporosarcina is a genus of gram-positive, rod-shaped bacteria that are commonly found in soil and water environments. These bacteria are known for their ability to produce spores, which are specialized cells that allow them to survive in harsh conditions and disperse to new locations. The morphology, or physical characteristics, of Sporosarcina bacteria can vary depending on the species and the conditions in which they are grown.

One key feature of Sporosarcina bacteria is their rod-like shape, which ranges from straight to slightly curved. They are typically 1-5 micrometers in length and 0.5-1.0 micrometers in diameter. The cell walls of Sporosarcina bacteria are thick and contain a high amount of peptidoglycan, which gives them a gram-positive stain when examined under a microscope.

In addition to their rod-like shape, Sporosarcina bacteria also have several other notable features. They are often found in clusters or chains, and may produce a slimy or mucoid exopolysaccharide layer on their surface. This layer helps the bacteria adhere to surfaces and form biofilms, which can protect them from environmental stresses.

Sporosarcina bacteria are also known for their ability to produce spores, which are highly resistant to environmental stresses such as heat, drying, and radiation. These spores are produced inside a special structure called the sporangium, which is located at the end of the bacterial cell. When the conditions become unfavorable for the bacteria, the sporangium ruptures and releases the spores, which can then germinate and grow into new cells once the conditions improve.

In addition to their morphological features, Sporosarcina bacteria have several other characteristics that make them interesting to researchers. They are often found in soil and water environments, and are known to be involved in the nitrogen cycle by converting nitrogen gas into a form that can be used by plants. Some species of Sporosarcina are also known to produce enzymes that can break down hydrocarbons, making them of interest for bioremediation efforts.

Overall, the morphology of Sporosarcina bacteria is characterized by their rod-like shape, ability to produce spores and biofilms, and presence in various environmental niches. Understanding the morphological and physiological features of these bacteria can help researchers better understand their role in the environment and potential applications in fields such as bioremediation and agriculture.

ABIS Encyclopedia

sporosarcina morphology

The cocci-shaped Sporosarcina genome on average contained 2. Antonie Van Leeuwenhoek 106:489—495. Examination of spore genes found the cocci-shaped Sporosarcina strains were lacking many genes that are found in Bacillus; however, spore genes that are present are well conserved among all the strains. Note, that the NBC-7SP live setup gives us an positive control where Ca +2 ions were present in same concentration 25 mM CaCl 2 as in NBUC-7SP live set. Two Gram-positive, endospore-forming, rod-shaped bacterial strains, HG645 T and HG711, were respectively isolated from surface water of a brackish lake and sediment of a fishery harbour in Japan and were subsequently characterized taxonomically using a polyphasic approach. Full size image Presence of mobile genetic elements was variable between the genomes depending on the type of element.

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Real

sporosarcina morphology

Pregerson originally identified each isolate as S. A plausible mechanism is schematically represented Figure B in S. All the tests undertaken in the lab were being performed for the first time by someone with no previous experience. Copy to Clipboard Reference Copied to Clipboard. Strains that lacked a very close phylogenetic neighbor tended to have a larger pool of strain-specific genes, for example strain P13, which forms its own clade, has 213 strain-specific genes or 6. A pH indicator dye 10 μL of phenol red was added to all the control and test sets after 24 hours of incubation to visualize the pH change. In fact, we have shown that, although they share morphological, biochemical, and 16S rDNA similarity, they are remarkably variable in their gene content, genome sequence identity, and methylomes.

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Sporosarcina Bacteria Identification

sporosarcina morphology

The DNA G+C content of strains HG645 T and HG711 were respectively 46. In the soil, nitrogen is very limited for plants, bacteria and other microbes, therefore there is a high level of competition for available nitrogen. All the liquid cultures were incubated in aerobic conditions at 30°C with an orbital shaker operated at 120 rpm. Thus, we see that the issue of the role of bacterial cells on mineral precipitation in MICP remains controversial and detailed studies delineating the exact mechanism leading to the bacterial cell surface deposition of CaCO 3 in in-situ conditions are necessary. Appl Microbiol Biotechnol 68:580—587. All strains were also placed in the exact eight clades, but there were minor modifications as to the relationship within the clade Additional file The core and pan-genomes of cocci-shaped strains of Sporosarcina.

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Sporosarcina saromensis sp. nov., an aerobic endospore

sporosarcina morphology

Conclusions This study begins to unravel the phylogenetic relationship of cocci-shaped Sporosarcina strains, and the comparative genomics of these strains supports identification of several new species. Whereas, the cocci-shaped Sporosarcina genomes ranged from 2 to 46 insertion sequence IS elements, with an average of 15. Calcium carbonate precipitation by Bacillus and Sporosarcina strains isolated from concrete and analysis of the bacterial community of concrete. The bacteria inoculated columns along with control sets were incubated at 30°C for a maximum duration of 7 days. The agar-cloumn samples were fixed with glutaraldehyde allowing us to study the MICP phenomenon in-situ.

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Taxonomic and genomic characterization of Sporosarcina cyprini sp. nov., moderately tolerant of Cr+6 and Cd+2 isolated from the gut of invasive fish Cyprinus carpio var. communis (Linn., 1758)

sporosarcina morphology

One of the large roles of Sporosarcina in the soil is the production of urease, which catalyzes the production of CO2 and ammonia, lowering the pH of surrounding soils and causing the precipitation of mineral ions Acha et al. They found rapid changes in the pH and Ca +2 concentrations near within 600 μm the biofilm during the MICP process. The addition of urea to the soil can have a detrimental effect on germinating seedlings and young plants, as well as populations of microorganisms Omar and Ismail 1999 , and species like S. Int J Syst Evol Microbiol 57:1694—1698. Ultimately, the global distribution of cocci-shaped Sporosarcina strains appears to be similar to the genus Sporosarcina as a whole. The bacterium Sporosarcina pasteurii SP is known for its ability to cause the phenomenon of microbially induced calcium carbonate precipitation MICP. Low intensity vaterite peaks o, 31.

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Sporosarcina thermotolerans

sporosarcina morphology

We investigated the micro and macroenvironment of MICP and conclusively demonstrated the presence of nanometer-sized crystals of CaCO 3 on the bacterial cell surfaces. In fact, no motif was common to all six strains and only one motif TTCGAA of 31 determined by PacBio sequencing, is shared between P33 and P37. Examining the genomics of phenotypically different and geographically diverse cocci-shaped Sporosarcina strains; we found an average genome size of 3. All the chemicals were used as purchased and solutions were prepared in Milli Q 18. In the control set NBC-7SP live without urea , bacterial activity was unable to change medium pH and also no precipitation was observed even in the presence of same molar concentration of Ca +2 as NBUC-7SP live. To the address the fundamental mechanism of MICP by S. DNA—DNA relatedness between strain HG645 T and the type strain of the phylogenetically related species Sporosarcina aquimarina was less than 10 %.

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Sporosarcina contaminans

sporosarcina morphology

Both core and accessory gene diversity failed to correlate with the nutritional growth requirements or location of isolation for the strains. The change in medium pH due to urease activity was supported by the urease enzyme supplemented NBUC-7urease test live and NBUC-9SP live sets see also Figure A in Fig 2. Name and taxonomic classification Last LPSN update 07-12-2022 DD-MM-YYYY Domain Bacteria" Phylum Bacillota Class Bacilli Order Caryophanales Family Caryophanaceae Genus Sporosarcina Species Sporosarcina soli Full Scientific Name PNU Sporosarcina soli Kwon et al. During the study, over 50 isolates of cocci-shaped Sporosarcina strains were collected from numerous locations around the world, including four different continents. For the purpose of obtaining more precise results, it is significant if several cultures are prepared in test media and duplicate performance of the tests themselves Gopal et al. Overall, all the strains within a clade share the 95% AAI minimum for identical species, but none of the clades share the 95% minimum between them Fig. On the basis of phylogenetic analysis and physiological and chemotaxonomic data, the isolates represent a novel species of the genus Sporosarcina, for which the name Sporosarcina saromensis sp.

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Sporosarcina newyorkensis 6062

sporosarcina morphology

Note that changes will be reviewed and judged. Genomic analysis revealed that cocci-shaped Sporosarcina strains contained an average genome of 3. Both the pH and the Ca 2+ concentration profiles were extended up to 600 μm from the biofilm surface, whereas the bulk chemical composition of the brine solution remained constant over the entire 4 h of SECM experiments. In this exercise of environmental Isolate, microorganisms are isolated from their natural habitat and identified through several series of bacterial culture tests. To date the only cocci-shaped Sporosarcina genome is a single draft genome of S.

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